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KMID : 0357119940160020171
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Korean Journal of Immunology
1994 Volume.16 No. 2 p.171 ~ p.176
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Immunodetection of Transforming Growth Factor-β-1 by an Improved Enzyme-Linked Immunosorbent Assay
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김윤정
이민철/박영의/김현태/최은영/변우현/김평현
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Abstract
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transforming growth factor-βs(TGF-βs) are highly conserveative family of 25 KD, homodimeric peptides. Among TGF-βs TGF-β1 is a prototype that has a broad range of biological activities including stimulation or inhibition of cellular
proliferation
and differentiation depending on target cell type and experimental conditions. Although sensitive bioassays for TGF-β1 have been described, their interference by other growth modulators have limited their application. Recently, the sandwich
enzymelined
immunosorbent assays (ELISAs) were developed for the detection of TGF-β1 in complex biological fluids. However, the preiusly developed ELISAs are based on use of a polyclonal rabbit anti-TGF-β1 which was limited by the availability of this
rather
low-titer antibody. This report describes a further improved TGF-β1 ELISA with comparable sevsitivities and specificities to the assays reported until the present time. This refined ELISA is the most sensitive, reproducible, and time-saving
method
for
the quantitation of TGF-β1.
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KEYWORD
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